Table of Contents

 

 

 

 

Acknowledgements

xi

 

Introduction

1

PART A

HUMAN REMAINS: DECAY, DNA, TISSUES AND FLUIDS

9

Chapter One

The Decay, Discovery and Recovery of Human Bodies

11

 

The Dead Body

11

 

The Stages of Decomposition

12

 

Factors Affecting the Speed of Decay

28

 

Discovery and Recovery of Human Remains

35

 

Determining the Age and Provenance of Skeletonized Remains

39

 

Future Developments

41

Chapter Two

Body Fluids and Waste Products

45

 

Blood Cells and Blood Typing

45

 

Methods for Detecting Blood

48

 

Confirming the Presence of Blood

51

 

Bloodstain Pattern Analysis

51

 

Artificial Blood

70

 

Post Mortem Toxicological Analysis of Blood

71

 

Saliva and Semen

72

 

Vitreous Humor

75

 

Faeces and Urine as Forensic Indicators

77

 

Future Directions

82

Chapter Three

Molecular Biology

85

 

The Structure of DNA

86

 

DNA Sampling

87

 

DNA Profiling

88

 

Polymerase Chain Reaction

92

 

Short Tandem Repeat Markers

97

 

Single Nucleotide Polymorphism Markers

107

 

Determination of Ethnicity

108

 

Determination of Physical Appearance

109

 

Determination of Personality Traits

110

 

Mobile Element Insertion Polymorphisms

110

 

Mitochondrial DNA

112

 

RNA

115

 

DNA Databases

116

 

Future Developments

120

Chapter Four

Human Tissues

123

 

The Outer Body Surface

123

 

Hair

138

 

Bones

142

 

Teeth

151

 

Future Developments

159

Chapter Five

Wounds

163

 

Definitions

163

 

Blunt Force Injuries

165

 

Sharp Force Traumas

172

 

Bone Damage

180

 

Additional Aspects of Wound Interpretation

183

 

Asphyxia

184

 

Pathology Associated with Drug Use

190

 

Gunshot Wounds

192

 

Bite Marks

200

 

Burns and Scalds

203

 

Ageing of Wounds

205

 

Post Mortem Injuries

207

 

Future Developments

209

PART B

INVERTEBRATES AND VERTEBRATES

211

Chapter Six

Invertebrates 1: Biological Aspects

213

 

An Introduction to Invertebrate Biology

213

 

Invertebrates as Forensic Indicators in Cases of Murder or Suspicious Death

214

 

Invertebrates as a Cause of Death

238

 

Invertebrates as Forensic Indicators in Cases of Neglect and Animal Welfare

241

 

The Role of Invertebrates in Food Spoilage and Hygiene Litigation

243

 

The Illegal Trade in Invertebrates

246

 

Invertebrate Identification Techniques

247

 

Future Directions

250

Chapter Seven

Invertebrates 2: Practical Aspects

253

 

Calculating the PMI/Time Since Infestation from Invertebrate Development Rates

254

 

Complicating Factors Affecting Earliest Oviposition Date Calculations

260

 

Determination of the PMI Using Invertebrate Species Composition

266

 

Determination of the PMI Using Ectoparasites

267

 

Determination of Movement from Invertebrate Evidence

267

 

Invertebrate Evidence in Cases of Wound Myiasis and Neglect

269

 

Detection of Drugs, Toxins and Other Chemicals in Invertebrates

271

 

Obtaining Human/Vertebrate DNA Evidence from Invertebrates

271

 

Determining the Source and Duration of Invertebrate Infestations of Food Products

272

 

Collecting Invertebrates for Forensic Analysis

273

 

Killing and Preserving Techniques for Invertebrates

276

 

Future Directions

279

Chapter Eight

Vertebrates

283

 

Introduction

284

 

Vertebrate Scavenging of Human Corpses

284

 

Vertebrates Causing Death and Injury

291

 

Neglect and Abuse of Vertebrates

292

 

Vertebrates and Drugs

293

 

Vertebrates and Food Hygiene

295

 

Illegal Trade and Killing of Protected Species of Vertebrates

295

 

Identification of Vertebrates

298

 

Future Directions

309

PART C

PROTISTS, FUNGI, PLANTS AND MICROBES

313

Chapter Nine

Protists, Fungi and Plants

315

 

Introduction

316

 

Protists

316

 

Fungi

321

 

Plants

324

 

Plant Secondary Metabolites as Sources of Drugs and Poisons

347

 

Illegal Trade in Protected Plant Species

351

 

Future Directions

353

Chapter Ten

Bacteria and Viruses

355

 

Introduction

355

 

The Role of Microorganisms in the Decomposition Process

356

 

Microbial Profiles as Identification Tools

357

 

Microbial Infections and Human Behaviour

370

 

Microbial Infections that can be Mistaken for Signs of Criminal Activity

372

 

The Use of Microorganisms in Bioterrorism

373

 

Future Directions

390

 

References

393

 

Index

417