PART 1

THE CLINICAL LABORATORY   Jay L. Bock, Matthew R. Pincus, Gregory A. Threatte

1

General Concepts and Administrative Issues   Anthony S. Kurec, Mark S. Lifshitz

3

2

Optimizing Laboratory Workflow and Performance   Thomas J. Dilts, Richard A, McPherson

13

3

Preanalysis   Kimberly W. Sanford, Richard A. McPherson

24

4

Analysis: Principles of Instrumentation   Robert L. Sunheimer, Gregory A. Threatte, Matthew R. Pincus, Mark S. Lifshitz

37

5

Analysis: Clinical Laboratory Automation   Robert L. Sunheimer, Mark S. Lifshitz, Gregory A. Threatte

64

6

Point-of-Care and Physician Office Laboratories   Gregory A. Threatte, Katherine I. Schexneider

73

7

Postanalysis: Medical Decision Making   Jeffrey S. Jhang, Anthony N. Sireci, Alexander Kratz

80

8

Interpreting Laboratory Results   Matthew R. Pincus, Naif Z. Abraham Jr.

91

9

Laboratory Statistics   Richard A. McPherson

109

10

Quality Control   W. Greg Miller

119

11

Clinical Laboratory Informatics   Paul Tranchida, Jay L Bock, Martin H. Bluth

135

12

Financial Management   David S. Wilkinson, Thomas J. Dilts, William Woolf, Mark S. Lifshitz

142

13

Biological, Chemical, and Nuclear Terrorism: Role of the Laboratory   Philip M. Tierno Jr., Mark S. Lifshitz

155

PART 2

CLINICAL CHEMISTRY   Matthew R. Pincus, Jay L. Bock, Martin H. Bluth

14

Evaluation of Renal Function, Water, Electrolytes, and Acid-Base Balance   Man S. Oh

169

15

Biochemical Markers of Bone Metabolism   Katrin M. Klemm, Michael J. Klein

193

16

Carbohydrates   Mukhtar I. Khan, Ruth S. Weinstock

210

17

Lipids and Dyslipoproteinemia   James Soh, Joby Josekutty, M. Mahmood Hussain

226

18

Cardiac Injury, Atherosclerosis, and Thrombotic Disease   Jay L Bock

249

19

Specific Proteins   Richard A. McPherson

259

20

Clinical Enzymology   Matthew R. Pincus, Naif Z. Abraham Jr., Robert P. Carty

273

21

Evaluation of Liver Function   Matthew R. Pincus, Philip M. Tierno Jr., Maly Fenelus, Wilbur B. Bowne, Martin H. Bluth

296

22

Laboratory Diagnosis of Gastrointestinal and Pancreatic Disorders   Martin J. Salwen, Haseeb A. Siddiqi, Frank G. Gress, Wilbur B. Bowne

312

23

Toxicology and Therapeutic Drug Monitoring   Matthew R. Pincus, Naif Z. Abraham Jr.

329

24

Evaluation of Endocrine Function   Helena A. Guber, Amal F Farag

365

25

Reproductive Function and Pregnancy   Dorota Borawski, Martin H. Bluth

402

26

Vitamins and Trace Elements   Martin J. Salwen

417

27

Chemical Basis for Analyte Assays and Common Interferences   Matthew R. Pincus, Jay L. Bock, Ralph Rossi, Donghong Cai

429

PART 3

URINE AND OTHER BODV FLUIDS   Richard A. McPherson, Gregory A. Threatte, Matthew R. Pincus

28

Basic Examination of Urine   Richard A, McPherson, Jonathan Ben-Ezra

445

29

Cerebrospinal, Synovial, Serous Body Fluids, and Alternative Specimens   Donald S. Karcher, Richard A. McPherson

480

PART 4

HEMATOLOGY, COAGULATION, AND TRANSFUSION MEDICINE   Robert E. Hutchison, Richard A. McPherson, Katherine I. Schexneider

30

Basic Examination of Blood and Bone Marrow   Neerja Vajpayee, Susan S. Graham, Sylva Bern

509

31

Hematopoiesis   Sharad C. Mathur, Katherine I. Schexneider, Robert E. Hutchison

536

32

Erythrocytic Disorders   M. Tarek Elghetany, Katalin Banki

557

33

Leukocytic Disorders   Robert E. Hutchison, Katherine I. Schexneider

601

34

The Flow Cytometric Evaluation of Hematopoietic Neoplasia   Brent L. Wood, Michael J. Borowitz

656

35

Immunohematology   Laura Cooling, Theresa Downs

674

36

Transfusion Medicine   Robertson D. Davenport, Paul D. Mintz

731

37

Hemapheresis   Jeffrey L. Winters

746

38

Tissue Banking and Progenitor Cells   Charlene A. Hubbell, Lazaro Rosales

774

PART 5

HEMOSTASIS AND THROMBOSIS   Jonathan L. Miller

39

Coagulation and Fibrinolysis   Alvin H. Schmaier, Jonathan L Miller

785

40

Blood Platelets and von Willebrand Disease   Jonathan L Miller, A. Koneti Rao

801

41

Laboratory Approach to Thrombotic Risk   Richard A. Marlar, Louis M. Fink, Jonathan L Miller

823

42

Antithrombotic Therapy   Louis M. Fink, Richard A. Marlar, Jonathan L. Miller

831

PART 6

IMMUNOLOGY AND IMMUNOPATHOLOGY   H. Davis Massey, Richard A. McPherson

43

Overview of the Immune System and Immunologic Disorders   Richard A. McPherson, H. Davis Massey

845

44

Immunoassays and Immunochemistry   Yoshihiro Ashihara, Yasushi Kasahara, Robert M. Nakamura

851

45

Laboratory Evaluation of the Cellular Immune System   Roger S. Riley, Ronald Mageau, Jonathan Ben-Ezra

877

46

Laboratory Evaluation of Immunoglobulin Function and Humoral Immunity    Richard A. McPherson, H. Davis Massey

899

47

Mediators of Inflammation: Complement, Cytokines, and Adhesion Molecules   H. Davis Massey, Richard A. McPherson

914

48

Human Leukocyte Antigen: The Major Histocompatibility Complex of Man   Omar R. Fagoaga

933

49

The Major Histocompatibility Complex and Disease   Julio C. Delgado

954

50

Immunodeficiency Disorders   Kimberly W. Sanford, Susan D. Roseff

963

51

Clinical and Laboratory Evaluation of Systemic Rheumatic Diseases   Carlos Alberto von Mühlen, Robert M, Nakamura

973

52

Vasculitis   Ankoor Shah, David J. Bylund, Rex M. McCallum

991

53

Organ-Specific Autoimmune Diseases   David J. Bylund, Robert M. Nakamura

1003

54

Allergic Diseases   Henry A. Homburger

1021

PART 7

MEDICAL MICROBIOLOGY   Gail L. Woods, Richard A. McPherson

55

Viral Infections   Michael Costello, Linda M. Sabatini, Margaret Yungbluth

1037

56

Chlamydial, Rickettsial, and Mycoplasmal Infections   David H. Walker, Gail L Woods, Michael B. Smith

1065

57

Medical Bacteriology   Geraldine S. Hall, Gail L Woods

1079

58

In Vitro Testing of Antimicrobial Agents   Michael B. Smith, P. Rocco LaSala, Gail L Woods

1116

59

Spirochete Infections   P. Rocco LaSala, Michael B. Smith

1129

60

Mycobacteria   Gail L. Woods

1145

61

Mycotic Diseases   Peter C. Iwen

1155

62

Medical Parasitology   Thomas R. Fritsche, Rangaraj Selvarangan

1188

63

Specimen Collection and Handling for Diagnosis of Infectious Diseases   Ann C. Croft, Gail L Woods

1239

PART 8

MOLECULAR PATHOLOGY   Martin H. Bluth, Elizabeth R. Unger, Matthew R. Pincus

64

Introduction to Molecular Pathology   Elizabeth R. Unger, Martin H. Bluth, Matthew R. Pincus

1255

65

Molecular Diagnostics: Basic Principles and Techniques   Martin Steinau, Margaret A. Piper, Elizabeth R. Unger

1258

66

Polymerase Chain Reaction and Other Nucleic Acid Amplification Technology   Frederick S. Nolte, Charles E. Hill

1271

67

Hybridization Array Technologies   Martin H. Bluth

1282

68

Applications of Cytogenetics in Modern Pathology   Constance K. Stein

1290

69

Establishing a Molecular Diagnostics Laboratory   Andrea Ferreira-Gonzalez, David S. Wilkinson, Martin H. Bluth

1314

70

Molecular Diagnosis of Genetic Diseases   Wayne W. Grody

1330

71

Identity Analysis: Use of DNA Analysis in Parentage, Forensic, and Missing Persons Testing   Herbert F. Polesky, Rhonda K. Roby

1348

72

Pharmacogenomics and Personalized Medicine   Jing Li, Martin H. Bluth, Andrea Ferreira-Gonzalez

1359

PART 9

CLINICAL PATHOLOGY OF CANCER   Matthew R. Pincus, Martin H. Bluth, Richard A. McPherson

73

Diagnosis and Management of Cancer Using Serologic and Tissue Tumor Markers   Peng Lee, Shilpa Jain, Wilbur B. Bowne, Matthew R. Pincus, Richard A. McPherson

1385

74

Oncoproteins and Early Tumor Detection   Matthew R. Pincus, Paul W. Brandt-Rauf, Martin H. Bluth, Wilbur B. Bowne

1400

75

Molecular Diagnosis of Hematopoietic Neoplasms   David R. Czuchlewski, David S. Viswanatha, Richard S. Larson

1415

76

Molecular Genetic Pathology of Solid Tumors   Peng Lee, Shilpa Jain, Matthew R. Pincus, Ruliang Xu

1441

77

High-Throughput Genomic and Proteomic Technologies in the Post-Genomic Era   Martin H. Bluth

1463

APPENDIXES   Richard A. McPherson, Matthew R. Pincus, Naif Z. Abraham Jr.

1

Physiologic Solutions, Buffers, Acid-Base Indicators, Standard Reference Materials, and Temperature Conversions

1481

2

Desirable Weights, Body Surface Area, and Body Mass Index

1485

3

Approximations of Total Blood Volume

1489

4

Periodic Table of the Elements

1490

5

SI Units

1491

6

Common Chimeric Genes Identified in Human Malignancies   Peng Lee, Naif Z. Abraham Jr., Matthew R. Pincus

1509

7

Disease/Organ Panels

1513