PART I

GENES AND CHROMOSOMES

1

Chapter 1

Genes Are DNA

2

Chapter 2

Genes Encode RNAs and Polypeptides

29

Chapter 3

Methods in Molecular Biology and Genetic Engineering

51

Chapter 4

The Interrupted Gene

93

Chapter 5

The Content of the Genome

112

Chapter 6

Genome Sequences and Gene Number

138

Chapter 7

Clusters and Repeats

160

Chapter 8

Genome Evolution

182

Chapter 9

Chromosomes

214

Chapter 10

Chromatin

239

PART II

DNA REPLICATION AND RECOMBINATION

269

Chapter 11

Replication Is Connected to the Cell Cycle

270

Chapter 12

The Replicon: Initiation of Replication

291

Chapter 13

DNA Replication

308

Chapter 14

Extra chromosomal Replication

331

Chapter 15

Homologous and Site-Specific Recombination

353

Chapter 16

Repair Systems

381

Chapter 17

Transposable Elements and Retroviruses

408

Chapter 18

Somatic Recombination and Hypermutation in the Immune System

441

PART III

GENE EXPRESSION

468

Chapter 19

Prokaryotic Transcription

469

Chapter 20

Eukaryotic Transcription

503

Chapter 21

RNA Splicing and Processing

525

Chapter 22

mRNA Stability and Localization

560

Chapter 23

Catalytic RNA

585

Chapter 24

Translation

606

Chapter 25

Using the Genetic Code

638

PART IV

GENE REGULATION

663

Chapter 26

The Operon

664

Chapter 27

Phage Strategies

696

Chapter 28

Eukaryotic Transcription Regulation

723

Chapter 29

Epigenetic Effects Are Inherited

756

Chapter 30

Regulatory RNA

780

Glossary

803

Appendix

Answers to Even-Numbered End-of-Chapter Questions

828

Index

830