Preface

v

Contributors

ix

List of Color Plates

xiii

PART I

ISOLATION AND PROPAGATION OF CELL POPULATIONS

1

Isolation and Propagation of Glomerular Mesangial Cells   Paolo Menè and Antonella Stoppacciaro

1

2

Isolation and Primary Culture of Human Proximal Tubule Cells   David A. Vesey, Weier Qi, Xinming Chen, Carol A. Pollock, and David W. Johnson

19

3

Propagation and Culture of Renal Fibroblasts   Lauren Grimwood and Rosemary Masterson

25

PART II

ANIMAL MODELS

4

Small Animal Models of Kidney Disease: A Review   Tim D. Hewitson, Takahiko Ono, and Gavin J. Becker

39

PART III

TECHNIQUES AND APPLICATIONS

5

Measurement of Glomerular Filtration Rate in Conscious Mice   Zhonghua Qi and Matthew D. Breyer

59

6

Laser Capture Microdissection of Kidney Tissue   Robert P. Woroniecki and Erwin P. Bottinger

73

7

Quantitative Gene Expression Analysis in Kidney Tissues   Chris Tikellis, Philip Koh, Wendy Burns, and Phillip Kantharidis

83

8

In Vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy   A. Richard Kitching, Michael P. Kuligowski, and Michael J. Hickey

109

9

Using In Situ Hybridization to Localize Renal Gene Expression in Tissue Sections   Ian A. Darby, Alexis Desmoulière, and Tim D. Hewitson

119

10

Immuno and Lectin Histochemistry for Renal Light Microscopy   Tim D. Hewitson and Lauren Grimwood

133

11

Immuno and Lectin Histochemistry for Renal Electron Microscopy   Mitsuru Nakajima

149

12

Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia   Christian Rosenberger, Seymour Rosen, Alexander Paliege, and Samuel N. Heyman

161

13

Identification of Apoptosis in Kidney Tissue Sections   Glenda Gobe

175

14

Cell-Populated Floating Collagen Lattices: An In Vitro Model of Parenchymal Contraction   Kristen J. Kelynack

193

15

Mechanical Stretch-Induced Signal Transduction in Cultured Mesangial Cells   Joan Krepinsky

205

16

Determination of Collagen Content, Concentration, and Sub-Types in Kidney Tissue   Chrishan S. Samuel

223

17

SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue   Eleni Giannakis, Chrishan S. Samuel, Wee-Ming Boon, Mary Macris, Tim D. Hewitson, and John D. Wade

237

18

In Vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli   Yoshitsugu Takabatake, Yoshitaka Isaka, and Enyu Imai

251

Index

265